Breaking the resolution limit in light microscopy

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Breaking the resolution limit in light microscopy.

Fluorescent imaging microscopy has been an essential tool for biologists over many years, especially after the discovery of the green fluorescent protein and the possibility of tagging virtually every protein with it. In recent years dramatic enhancement of the level of detail at which a fluorescing structure of interest can be imaged have been achieved. We review classical and new developments...

متن کامل

k- Microscopy – resolution beyond the diffraction limit

We present a novel Fourier domain method for microscopic imaging – so-called kmicroscopy– with lateral resolution independent of the detection numerical aperture. The concept is based on sample illumination by a lateral fringe-pattern of varying spatial frequency, which probes the lateral spatial frequency or kspectrum of the sample structure. The illumination pattern is realized by interferenc...

متن کامل

Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT.

We present a plane-scanning RESOLFT [reversible saturable/switchable optical (fluorescence) transitions] light-sheet (LS) nanoscope, which fundamentally overcomes the diffraction barrier in the axial direction via confinement of the fluorescent molecular state to a sheet of subdiffraction thickness around the focal plane. To this end, reversibly switchable fluorophores located right above and b...

متن کامل

Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay exponentially with depth. Optical imaging beyond the ballistic regime has been demonstrated by hybrid techniques that combine light with the deeper penetration capabil...

متن کامل

Breaking the Resolution Limit in Medical Image Modalities

The image processing technique recognized as super-resolution have proven their valuable in reconstruction high-quality images from a low-resolution image sets. In this paper, author presents new hybrid SR algorithm for medical image processing. The algorithm combines both single and multi frame super-resolution frameworks. The technique derives advantages from rigoristic optical flow motion es...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Briefings in Functional Genomics and Proteomics

سال: 2006

ISSN: 1473-9550,1477-4062

DOI: 10.1093/bfgp/ell036